Osteoclast differentiation of bone marrow adherent cells

doi:10.3969/j.issn.2095-4344.2014.07.001

Abstract

Abstract:

BACKGROUND: Generally considered bone marrow cells obtained by adherent method are mesenchymal stem cells, and they can differentiate into osteoblasts, adipocytes and chondrocytes.
OBJECTIVE: To explore the differentiation capacity of bone marrow adherent cells into osteoclasts.
METHODS: Primary mouse mesenchymal stem cells were obtained using adherent method, and bone marrow cells were obtained through adherence 1-5 days. Both of them were cultured with normal medium and inducing medium containing m-csf and RANKL. After 9 days, cells were stained by alkaline phosphatase and tartrate-resistant acid phosphatase. The passage 2 mesenchymal stem cells were divided into four groups, which were induced by mock, m-csf, RANKL and m-csf+RANKL, respectively. After 9 days of culture, the cells were stained by alkaline phosphatase and tartrate-resistant acid phosphatase.
RESULTS AND CONCLUSION: Primary culture of adhered cells was uniform. Alkaline phosphatase and tartrate-resistant acid phosphatase staining of primary and passaged bone marrow adherent cells induced with m-csf+RANKL were positive. This result showed that there were cells that could be induced into osteoclasts in the primary and passaged bone marrow adherent cells. Alkaline phosphatase and tartrate-resistant acid phosphatase staining showed differences of adherent cells at different times after the induction, indicating that adherent cells at different times had different differentiation capacity.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, osteoclasts, cells, cultured

CLC Number:

R318

Chen Jing, Ma Ji, Sun Fen-yong. Osteoclast differentiation of bone marrow adherent cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 985-990.

Figures/Tables 4

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